Hypertension in Prenatally Undernourished Young-Adult Rats Is Maintained by Tonic Reciprocal Paraventricular–Coerulear Excitatory Interactions

Prenatally malnourished rats develop hypertension in adulthood, in part through increased α₁-adrenoceptor-mediated outflow from the paraventricular nucleus (PVN) to the sympathetic system. We studied whether both α₁-adrenoceptor-mediated noradrenergic excitatory pathways from the locus coeruleus (LC) to the PVN and their reciprocal excitatory CRFergic connections contribute to prenatal undernutrition-induced hypertension. For that purpose, we microinjected either α₁-adrenoceptor or CRH receptor agonists and/or antagonists in the PVN or the LC, respectively. We also determined the α₁-adrenoceptor density in whole hypothalamus and the expression levels of α_1A-adrenoceptor mRNA in the PVN. The results showed that: (i) agonists microinjection increased systolic blood pressure and heart rate in normotensive eutrophic rats, but not in prenatally malnourished subjects; (ii) antagonists microinjection reduced hypertension and tachycardia in undernourished rats, but not in eutrophic controls; (iii) in undernourished animals, antagonist administration to one nuclei allowed the agonists recover full efficacy in the complementary nucleus, inducing hypertension and tachycardia; (iv) early undernutrition did not modify the number of α₁-adrenoceptor binding sites in hypothalamus, but reduced the number of cells expressing α_1A-adrenoceptor mRNA in the PVN. These results support the hypothesis that systolic pressure and heart rate are increased by tonic reciprocal paraventricular–coerulear excitatory interactions in prenatally undernourished young-adult rats.     

The Protection of Lactic Acid Bacteria Fermented-Mango Peel against Neuronal Damage Induced by Amyloid-Beta

Mango peels are usually discarded as waste; however, they contain phytochemicals and could provide functional properties to food and promote human health. This study aimed to determine the optimal lactic acid bacteria for fermentation of mango peel and evaluate the effect of mango peel on neuronal protection in Neuron-2A cells against amyloid beta (Aβ) treatment (50 μM). Mango peel can be fermented by different lactic acid bacteria species. Lactobacillus acidophilus (BCRC14079)-fermented mango peel produced the highest concentration of lactic acid bacteria (exceeding 10⁸ CFU/mL). Mango peel and fermented mango peel extracts upregulated brain-derived neurotrophic factor (BDNF) expression for 1.74-fold in Neuron-2A cells. Furthermore, mango peel fermented products attenuated oxidative stress in Aβ-treated neural cells by 27%. Extracts of L. acidophilus (BCRC14079)-fermented mango peel treatment decreased Aβ accumulation and attenuated the increase of subG1 caused by Aβ induction in Neuron-2A cells. In conclusion, L. acidophilus (BCRC14079)-fermented mango peel acts as a novel neuronal protective product by inhibiting oxidative stress and increasing BDNF expression in neural cells.     

Interactions of DNA with graphene and sensing applications of graphene field-effect transistor devices: A review

Graphene field-effect transistors (GFET) have emerged as powerful detection platforms enabled by the advent of chemical vapor deposition (CVD) production of the unique atomically thin 2D material on a large scale. DNA aptamers, short target-specific oligonucleotides, are excellent sensor moieties for GFETs due to their strong affinity to graphene, relatively short chain-length, selectivity, and a high degree of analyte variability. However, the interaction between DNA and graphene is not fully understood, leading to questions about the structure of surface-bound DNA, including the morphology of DNA nanostructures and the nature of the electronic response seen from analyte binding. This review critically evaluates recent insights into the nature of the DNA graphene interaction and its affect on sensor viability for DNA, small molecules, and proteins with respect to previously established sensing methods. We first discuss the sorption of DNA to graphene to introduce the interactions and forces acting in DNA based GFET devices and how these forces can potentially affect the performance of increasingly popular DNA aptamers and even future DNA nanostructures as sensor substrates. Next, we discuss the novel use of GFETs to detect DNA and the underlying electronic phenomena that are typically used as benchmarks for characterizing the analyte response of these devices. Finally, we address the use of DNA aptamers to increase the selectivity of GFET sensors for small molecules and proteins and compare them with other, state of the art, detection methods.     

“Parallel factor analysis of multi-excitation ultraviolet resonance Raman spectra for protein secondary structure determination”

Protein secondary structural analysis is important for understanding the relationship between protein structure and function, or more importantly how changes in structure relate to loss of function. The structurally sensitive protein vibrational modes (amide I, II, III and S) in deep-ultraviolet resonance Raman (DUVRR) spectra resulting from the backbone C–O and N–H vibrations make DUVRR a potentially powerful tool for studying secondary structure changes. Experimental studies reveal that the position and intensity of the four amide modes in DUVRR spectra of proteins are largely correlated with the varying fractions of α-helix, β-sheet and disordered structural content of proteins. Employing multivariate calibration methods and DUVRR spectra of globular proteins with varying structural compositions, the secondary structure of a protein with unknown structure can be predicted. A disadvantage of multivariate calibration methods is the requirement of known concentration or spectral profiles. Second-order curve resolution methods, such as parallel factor analysis (PARAFAC), do not have such a requirement due to the “second-order advantage.” An exceptional feature of DUVRR spectroscopy is that DUVRR spectra are linearly dependent on both excitation wavelength and secondary structure composition. Thus, higher order data can be created by combining protein DUVRR spectra of several proteins collected at multiple excitation wavelengths to give multi-excitation ultraviolet resonance Raman data (ME-UVRR). PARAFAC has been used to analyze ME-UVRR data of nine proteins to resolve the pure spectral, excitation and compositional profiles. A three factor model with non-negativity constraints produced three unique factors that were correlated with the relative abundance of helical, β-sheet and poly-proline II dihedral angles. This is the first empirical evidence that the typically resolved “disordered” spectrum represents the better defined poly-proline II type structure.     

Experimental study on the fiber pull-out of composites using digital gradient sensing technique

A method to measure the stress field at the fiber tip in the fiber pull out test was proposed by using a digital gradient sensing technique. First, the principle of digital gradient sensing is introduced, and the non-contact optical system of digital gradient sensing developed. Then, a fiber reinforced composite model specimen, where a nail was inserted in epoxy resin to act as a fiber, was performed, and a pull out test was conducted on the specimen using the digital gradient sensing technique. Finally, the angular deflections contour at the fiber tip was obtained, and the stress intensity factor was extracted from the angular deflections. The results show that the stress intensity factor at the fiber tip extracted from the angular deflections agreed with the results calculated by the finite element method.     

One‐Pot Synthesis of Aliphatic Nitro Compounds by Michael/retro‐Claisen Fragmentation Domino Reaction

A novel and concise method for the construction of γ‐nitro ketones and 1,3‐dinitro compounds with chemoselective acyl transfer has been presented under basic conditions via one‐pot reactions. These reactions feature high atom‐ and step‐economic possess, mild condition and simple operation for one‐pot synthesis of aliphatic nitro compounds. This protocol provides the products in good yields. The possible reaction mechanism has also been proposed.     

Nannocystin A: an Elongation Factor 1 Inhibitor from Myxobacteria with Differential Anti‐Cancer Properties

Cultivation of myxobacteria of the Nannocystis genus led to the isolation and structure elucidation of a class of novel cyclic lactone inhibitors of elongation factor 1. Whole genome sequence analysis and annotation enabled identification of the putative biosynthetic cluster and synthesis process. In biological assays the compounds displayed anti‐fungal and cytotoxic activity. Combined genetic and proteomic approaches identified the eukaryotic translation elongation factor 1α (EF‐1α) as the primary target for this compound class. Nannocystin A ( 1 ) displayed differential activity across various cancer cell lines and EEF1A1 expression levels appear to be the main differentiating factor. Biochemical and genetic evidence support an overlapping binding site of 1 with the anti‐cancer compound didemnin B on EF‐1α. This myxobacterial chemotype thus offers an interesting starting point for further investigations of the potential of therapeutics targeting elongation factor 1.     

结构动力学方程的二次加速度积分法

本文提出了结构动力学方程求解的一类二次加速度逐步积分法，推导了计算公式，分析了积分稳定性和精度。通过理论分析和具体算例表明，这种方法具有相当高的积分精度，但积分是条件稳定的。     

Liquid single- and gas—liquid two-phase flows of magnetic fluid in the presence of a high non-uniform parallel magnetic field

The effect of a non-uniform parallel high magnetic field on flow control characteristics is investigated experimentally for a magnetic fluid single-phase flow and an air—magnetic fluid two-phase flow in a vertical channel. It is found that as the magnetic field strength is increased, the friction factor of the single-phase flow increases significantly. For the two-phase flow, the friction pressure loss and the head pressure loss, which is smaller than the friction loss, are negligibly small compared with the magnetic pressure loss. In the case where air is injected 27.9d upstream from the maximum magnetic field, the air flow is blocked by the magnetic force in the entrance of the magnetic field, which leads to increases in both local void fraction and pressure drop there. In the case where air is injected 1.43d downstream from the maximum magnetic field, the air flow is accelerated, resulting in a decrease in void fraction and an increase in pressure rise. In the latter case and under the present range of experimental conditions, the magnetic pumping head reaches 0.02 MPa at the highest, and the maximum circulation flow rate reaches twice as high as non-magnetically driven flow rate.     

双周期裂纹压电材料反平面剪切问题断裂力学分析

研究压电材料双周期裂纹反平面剪切与平面电场作用的问题．运用复变函数方法，获得了该问题严格的闭合解，并由此给出了裂纹尖端应力强度因子和电位移强度因子的精确公式．数值算例显示了裂纹分布特征对材料断裂行为的重要影响．叠间小裂纹能够对主裂纹的应力和电位移场起着屏蔽作用，相反行间小裂纹却起着放大作用，至于钻石形分布裂纹的影响规律则更为复杂．对于某些特殊情形给予了解答并导出一系列有意义的结果。